The money will be used to help each city deliver plans to get more people cycling by improving and expanding cycle routes between the city centre, local communities and key employment and retail areas.
The money will be allocated as follows: Birmingham, Leeds and Manchester (all £22m), Bristol (£19m), Newcastle (£10.6m), Norwich (£8.4m), Cambridge (£6m) and Oxford (£3.3m).
Nick Clegg, deputy prime minister, said: “We are in the midst of a cycling revolution in the UK but we need to make sure we’re in the right gear to see it through.
“With the legacy of the 2012 Olympics and the Tour de France in Yorkshire last year still fresh in our minds, this money can help Britain become a cycling nation to rival the likes of Denmark and the Netherlands.
“Research shows that boosting cycling could save billions of pounds otherwise spent on the NHS, reduce pollution and congestion, and create a happier and safer population.”
Robert Goodwill, transport minister with responsibility for cycling, said: “Cycling is great for your health and good for the environment, and this Government is doing all it can to help more people get out on their bikes.
“We have doubled the amount of money available for cycling and taken steps to make sure that future Governments plan properly for cycling.”